Building the Right Workflow for Your Laboratory 

The ideal CLD workflow will look different for every laboratory. Available space, throughput, level of automation, application requirements, and future plans can all influence which technologies are the best fit.

At BPES, we bring together a curated portfolio of specialist technologies and application expertise to help researchers build CLD workflows around these requirements. Rather than expecting one platform to meet every need, we work with researchers to identify the right technology for each stage and consider how the different elements can fit together within the wider workflow.

For some laboratories, increasing throughput and reducing manual intervention may make automation a priority. For others, instrument footprint, flexibility, or specific application capabilities may be more important. Considering these factors can help create a workflow that meets current needs while allowing the programme to develop.

Helping Researchers Build More Efficient CLD Workflows 

Developing a cell line is a journey through multiple stages, each with its own requirements. From isolating individual cells and establishing clonality to monitoring developing clones, assessing performance, and moving towards larger-scale culture, researchers need reliable tools and data to make decisions along the way.

The right technologies can help make these processes more efficient, consistent, and scalable. However, with different needs at different stages, there is rarely a single solution that does everything equally well.

We are pleased to bring together a portfolio of specialist technologies to support key stages of the CLD workflow. Together, they provide options for building a workflow around your laboratory's requirements, from the first individual cell through to early-stage expansion.

Our portfolio spans key stages of the CLD workflow, from the first individual cell through to early-stage expansion, giving researchers the flexibility to build a workflow suited to their laboratory.

Starting with Single-Cell Isolation 

Every clonal cell line starts with an individual cell. Efficiently isolating and depositing single cells into multi-well plates provides the foundation for downstream clonality assessment and clone development. 

To support this step, the SEED Biosciences DispenCell-S4 uses impedance-based single-cell dispensing for efficient isolation, with high single-cell occupancy and a high probability of monoclonality. Low-pressure dispensing and an automation-ready design can also support greater consistency and throughput at this early stage.

Establishing and Monitoring Clonality

Imaging provides a way to monitor developing clones over time, from the initial single cell through to colony formation. By capturing this progression, researchers can generate visual evidence supporting clonality while also revealing changes in colony formation, morphology, confluence, and cell growth.

The SYNENTEC CELLAVISTA and NYONE imaging systems provide automated image acquisition and analysis for a range of cell-based applications. Within CLD workflows, they can support clonality assessment, colony monitoring, cell counting, confluence analysis, and morphology assessment, helping researchers make consistent, data-driven decisions throughout clone development.

Combining SEED's impedance-based single-cell dispensing with SYNENTEC's image-based monitoring provides complementary evidence of clonality: confirming the presence of a single cell at dispensing and documenting its subsequent development into a colony. This dual evidence approach supports the level of clonality documentation expected in regulated CLD.

Putting Clone Performance to the Test 

As promising clones emerge, understanding how they perform under different culture conditions becomes increasingly important. Testing multiple conditions in parallel can help researchers understand clone behaviour before progressing further.

Leadgene Biosolutions' C.BIRD, C.NEST 2.0 and S.NEST 2.0 provide microbioreactor-based systems for parallel culture experiments. The NEST platforms support defined mixing and high-throughput experimentation in multi-well formats, while the S.NEST 2.0 additionally provides integrated pH and dissolved oxygen monitoring. 

Moving Towards Larger-Scale Culture 

As promising clones progress, researchers need to move beyond small-scale culture while maintaining appropriate growth conditions.

Thomson Instrument Company's Optimum Growth flasks and plates support this transition with specialised culture vessels designed to promote consistent aeration and growth. They provide a practical intermediate step between multi-well culture and subsequent shake-flask or upstream processing.

Supporting Your CLD Journey 

We combine specialist technologies with application-focused support at BPES to help researchers build CLD workflows around their specific requirements. We can help you identify the right technologies for each stage while considering factors such as throughput, available space, automation, and future development.

Whether you're looking to optimise an existing process, introduce new capabilities, or build a CLD workflow from the ground up, we can help you develop an approach that fits your laboratory and supports your goals at each stage of cell line development.

Explore our specialist CLD portfolio in practice. Get in touch with our team to arrange a demonstration and discuss how the technologies could fit into your workflow.