Description
Plasmid+® liquid media is an enriched media specifically designed for
plasmid DNA production. Plasmid+® supports much higher cell densities
and plasmid yields than LB media. Optimal shake flask yields are achieved
using Ultra Yield® Flask and vented cap, which facilitates maximum culture aeration. Plasmid+® media may also be used in a bioreactor with continuous aeration and agitation.
Advantages
- Medium specifically formulated for high DNA growth
- Increased supercoiled DNA per Liter
- Consistent plasmid production up to 22 hours
- Use with tubes, plates, flasks, and fermenters
- Sterile and ready to use. Simply add antibiotics and Grow!
- Store Plasmid+® liquid media at room temperature for up to 24 months
- Animal Origin Free Formulation
Increased Efficiency
The following diagram illustrates the increased efficiency in plasmid production when using Plasmid+® media over conventional media. A plasmid yield of 2.5mg was achieved when using either a 2.5L Ultra Yield® flask with 500mL of conventional media or a 250mL Ultra Yield® flask with 60mL of Plasmid+® media. No need to use large flasks and lots of media when you can get by with 8x less media to generate the same results.
Improved DNA Protocol
Four E. coli constructs were grown in either LB media or Plasmid+® media in 250mL Ultra Yield® Flasks using optimal fill volumes and shake speeds. After 24 hours in culture each flask was harvested and plasmid yields compared. As shown, Plasmid+® media greatly improved plasmid yields in all cultures on average over 1900% compared with LB Media.
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More on Thomson Instruments
Thomson Instrument Company has been serving the pharmaceutical and life science industries since 1970. Their lasting commitment to provide customer satisfaction and quality products has enabled them to maintain a solid reputation throughout the industry. Thomson’s range of innovate consumables and equipment help make your lab more efficient. Their filter vials speed up prep and analysis, while their patented Optimum Growth and Ultra High Yield Flasks with AirOTop seals provide the perfect environment to enhance cell growth. Their range of well plates represent a cross section of plates for many different needs.
